Dergi
/
Biyokimya
/
毛细管电泳与亲和毛细管电泳对金属离子 AtHIRD11 固有紊乱与结合的分析
JoVE Journal
Biyokimya
Bu içeriği görüntülemek için JoVE aboneliği gereklidir.  Oturum açın veya ücretsiz deneme sürümünü başlatın.
JoVE Journal Biyokimya
Analysis of AtHIRD11 Intrinsic Disorder and Binding Towards Metal Ions by Capillary Gel Electrophoresis and Affinity Capillary Electrophoresis
Please note that all translations are automatically generated. Click here for the English version.

毛细管电泳与亲和毛细管电泳对金属离子 AtHIRD11 固有紊乱与结合的分析

5,852 Views

•

07:54 min

•

August 22, 2018

DOI:

10.3791/57749-v

DOI:
10.3791/57749-v

•

07:54 min
August 22, 2018

•

2 Views
, , ,
1Institute of Medicinal and Pharmaceutical Chemistry,TU Braunschweig

Özet

Automatically generated

该协议结合毛细管电泳对蛋白质样品的特性进行了表征, 并对带电配体进行了快速结合筛选。推荐用于具有弹性结构的蛋白质, 如内在紊乱的蛋白质, 以确定不同构象的结合的差异。

Explore More Videos

Intrinsic DisorderMetal Ion BindingCapillary Gel ElectrophoresisAffinity Capillary ElectrophoresisConformational ChangesGlass CapillaryPolyamide CoatingDetection WindowCalcium ChlorideMetal Salt SolutionsTris BufferAcetanilide EOF MarkerSample SolutionHydrodynamic InjectionElectrophoretic Separation

Read Article

Cite this Article

Nachbar, M., Maul, J., Stein, M., Wätzig, H. Analysis of AtHIRD11 Intrinsic Disorder and Binding Towards Metal Ions by Capillary Gel Electrophoresis and Affinity Capillary Electrophoresis. J. Vis. Exp. (138), e57749, doi:10.3791/57749 (2018).

Download .ris file

Copy

Share Video

.

Copy