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A11-positiver β-Amyloid Oligomer Vorbereitung und Bewertung mit Dot-Blot-Analyse
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06:17 min
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May 22, 2018
DOI:
1Ningbo Key Laboratory of Behavioral Neuroscience, Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine,Ningbo University, 2Li Dak Sum Yip Yio Chin Kenneth Li Marine Biopharmaceutical Research Center,Ningbo University, 3School of Materials Science and Chemical Engineering,Ningbo University
Özet
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Dieses Protokoll beschreibt, wie Aβ Oligomere aus einem synthetischen Peptid in Vitro vorzubereiten und relative Mengen der Aβ Oligomer durch eine Dot-Blot-Analyse zu bewerten.
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Chunhui, H., Dilin, X., Ke, Z., Jieyi, S., Sicheng, Y., Dapeng, W., Qinwen, W., Wei, C. A11-positive β-amyloid Oligomer Preparation and Assessment Using Dot Blotting Analysis. J. Vis. Exp. (135), e57592, doi:10.3791/57592 (2018).
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