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CARIP 和芯片序列: 鉴定胚胎干细胞中染色质相关 rna 和蛋白质-DNA 相互作用的方法
JoVE Journal
Gelişim Biyolojisi
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JoVE Journal Gelişim Biyolojisi
CARIP-Seq and ChIP-Seq: Methods to Identify Chromatin-Associated RNAs and Protein-DNA Interactions in Embryonic Stem Cells
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CARIP 和芯片序列: 鉴定胚胎干细胞中染色质相关 rna 和蛋白质-DNA 相互作用的方法

DOI:
10.3791/57481-v

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11:13 min

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May 25, 2018

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1Department of Oncology,Wayne Eyalet Üniversitesi Tıp Fakültesi, 2Karmanos Cancer Institute,Wayne Eyalet Üniversitesi Tıp Fakültesi

Bölümler

  • 00:04Başlık
  • 01:02Crosslinking of Embryonic Stem Cells for ChIP and CARIP
  • 02:29Chromatin Sonication for ChIP and CARIP
  • 03:48ChIP and CARIP Process
  • 07:12Double-stranded cDNA Synthesis for CARIP-Seq
  • 08:53Use of ChIP-Seq and CARIP-Seq to Study Global Protein-DNA Interactions and Chromatin-Associated RNAs in Embryonic Stem Cells
  • 10:43Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

在这里, 我们描述了执行芯片序列和 CARIP 的方法, 包括用于下一代测序的库准备, 以生成 ES 细胞中的全球 epigenomic 和染色质相关的 RNA 图谱。

Etiketler

CARIP-SeqChIP-SeqChromatin-associated RNAsProtein-DNA InteractionsEmbryonic Stem CellsEkolojiTranscription FactorsEpigenetic RegulatorsHistone ModificationsChromatin ImmunoprecipitationSonicationFormaldehyde Cross-linkingGlycinePhosphate Buffered SalineTrypsinizationMEF MediumFetal Bovine SerumSonication BufferSodium Dodecyl Sulfate

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