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小鼠肝灌注肝细胞和正弦内皮细胞的纯化
JoVE Journal
Biyokimya
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JoVE Journal Biyokimya
Purification of Hepatocytes and Sinusoidal Endothelial Cells from Mouse Liver Perfusion
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小鼠肝灌注肝细胞和正弦内皮细胞的纯化

DOI:
10.3791/56993-v

•

08:54 min

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February 12, 2018

•

, , , , , ,
1Department of Biochemistry,University of Nebraska

Bölümler

  • 00:04Başlık
  • 00:45Catheter Placement
  • 02:46Liver Perfusion and Hepatocyte Purification
  • 04:39Sinusoidal Endothelial Cell (SEC) Purification
  • 07:18Results: Representative SEC Purification Analyses
  • 08:24Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

该协议的目的是获得高生存能力和高产量的肝细胞和正弦内皮细胞。这是通过灌注肝与 IV 型胶原酶溶液通过门静脉, 其次是差异离心获得肝细胞和正弦内皮细胞。

Etiketler

Mouse Liver PerfusionHepatocyte PurificationSinusoidal Endothelial Cell PurificationLiver Cell PopulationsPortal Vein CatheterizationLiver DigestionLiver Cell ViabilityHepatocyte HarvestingBuffer Solutions

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