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Medición de la deformabilidad y la heterogeneidad de glóbulos rojos en sangre por Ektacytometry
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JoVE Journal İmmünoloji ve Enfeksiyon
Measuring Deformability and Red Cell Heterogeneity in Blood by Ektacytometry
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Medición de la deformabilidad y la heterogeneidad de glóbulos rojos en sangre por Ektacytometry

14,196 Views

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09:12 min

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January 12, 2018

DOI:

10.3791/56910-v

DOI:
10.3791/56910-v

•

09:12 min
January 12, 2018

•

19 Views
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1Pediatri Anabilim Dalı,Saint Louis University School of Medicine, 2Molecular and Clinical Nutrition Section, Digestive Diseases Branch,National Institute of Diabetes and Digestive and Kidney Diseases, 3Molecular and Clinical Hematology Branch,National Institute of Diabetes and Digestive and Kidney Diseases, 4Sickle Cell Branch, National Heart, Lung and Blood Institute,National Institutes of Health, 5Edward A. Doisy Department of Biochemistry and Molecular Biology,Saint Louis University School of Medicine, 6Red Cell Physiology Laboratory,New York Blood Center

Özet

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Aquí presentamos técnicas para medir la deformabilidad del glóbulo rojo y la heterogeneidad celular por ektacytometry. Estas técnicas son aplicables a las investigaciones generales de la deformabilidad del glóbulo rojo y las investigaciones específicas de enfermedades de la sangre caracterizadas por la presencia de rígidos y deformables eritrocitos en la circulación, tales como anemia de células falciformes.

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EktacytometryRed Cell DeformabilityRed Cell HeterogeneitySickle Cell AnemiaDiabetesMicrovascular DiseasePVP SolutionDiffraction ImageData Acquisition

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Parrow, N. L., Violet, P., Tu, H., Nichols, J., Pittman, C. A., Fitzhugh, C., Fleming, R. E., Mohandas, N., Tisdale, J. F., Levine, M. Measuring Deformability and Red Cell Heterogeneity in Blood by Ektacytometry. J. Vis. Exp. (131), e56910, doi:10.3791/56910 (2018).

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