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An Engineered Split-TET2 Enzyme for Chemical-inducible DNA Hydroxymethylation and Epigenetic Remodeling
1Centre for Epigenetics and Disease Prevention, Department of Molecular and Cellular Medicine, Institute of Biosciences and Technology, College of Medicine,Texas A&M University, 2Centre for Translational Cancer Research, Department of Medical Physiology, Institute of Biosciences and Technology, College of Medicine,Texas A&M University
Bölümler
- 00:05Başlık
- 00:48Cell Culture, Plasmid Transfection, and Chemical Induction
- 01:45Quantification of Rapamycin-indiced 5-hydroxymethylcystosine (5hmc) Production by Immunostaining
- 03:08Dot-blot Assay Quantify 5hmc and 5-methylcystosine (5mc) Amounts
- 07:03Results: Validation of Chemically Induced 5hmc Levels
- 07:58Conclusion
