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雌性生殖道交配后精子的收集及对小鼠精子液化的测定
JoVE Journal
Gelişim Biyolojisi
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JoVE Journal Gelişim Biyolojisi
Collection of Post-mating Semen from the Female Reproductive Tract and Measurement of Semen Liquefaction in Mice
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雌性生殖道交配后精子的收集及对小鼠精子液化的测定

DOI:
10.3791/56670-v

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12:06 min

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November 18, 2017

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1School of Molecular Biosciences, Center for Reproductive Biology, College of Veterinary Medicine,Washington State University

Bölümler

  • 00:00Başlık
  • 02:02Mice, Mating, and Assessing Copulatory Plug
  • 03:25Preparation Before Tissue Collection
  • 04:40Tissue Collection
  • 07:11Measurement of Semen Liquefaction from the Uterine Content Collection
  • 09:25Sonuçlar
  • 10:02Example of Downstream Application: Video Imaging of Sperm Motility
  • 11:13Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

精子的液化需要从精凝胶中解放出来。本研究提供了从雌性生殖道 post-coitus 中收集精子, 以及测定精子液化时间的程序。

Etiketler

Post-mating SemenSemen LiquefactionMiceFemale Reproductive TractSemen CollectionSperm MotilityProstate Specific Antigen (PSA)InfertilityVaginal PlugLeibovitz’s L-15 MediaFetal Bovine Serum

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