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Einzellige Quantifizierung der Protein-Abbauraten von Time-Lapse Fluoreszenzmikroskopie in adhärente Zellkultur
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JoVE Journal Gelişim Biyolojisi
Single-Cell Quantification of Protein Degradation Rates by Time-Lapse Fluorescence Microscopy in Adherent Cell Culture
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Einzellige Quantifizierung der Protein-Abbauraten von Time-Lapse Fluoreszenzmikroskopie in adhärente Zellkultur

8,460 Views

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12:06 min

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February 04, 2018

DOI:

10.3791/56604-v

DOI:
10.3791/56604-v

•

12:06 min
February 04, 2018

•

7 Views
,
1UPSUTER, Institute of Bioengineering (IBI), School of Life Sciences,École Polytechnique Fédérale de Lausanne (EPFL)

Özet

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Dieses Protokoll beschreibt eine Methode zum Bestimmen von Protein in einzelne lebende adhärente Zellen, Halbwertszeiten mit Puls Kennzeichnung und Zeitraffer Fluoreszenz-Bildgebung von Fusionsproteinen SNAP-Tag.

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Alber, A. B., Suter, D. M. Single-Cell Quantification of Protein Degradation Rates by Time-Lapse Fluorescence Microscopy in Adherent Cell Culture. J. Vis. Exp. (132), e56604, doi:10.3791/56604 (2018).

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