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单细胞下游特征的靶细胞预富集与全基因组扩增
JoVE Journal
Kanser Araştırmaları
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JoVE Journal Kanser Araştırmaları
Target Cell Pre-enrichment and Whole Genome Amplification for Single Cell Downstream Characterization

单细胞下游特征的靶细胞预富集与全基因组扩增

DOI:

10:12 min

May 15, 2018

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Bölümler

  • 00:05Başlık
  • 01:28Preparation of Target Cells
  • 02:41Charging the Wire
  • 03:27Counting Cells on the Wire
  • 04:03Detachment and Recovery of Target Cells
  • 05:25Single-cell Sampling Using Micromanipulation
  • 06:37Single-cell Sampling Using Laser Microdissection
  • 07:47Results: Use of Functionalized Wire for Ex Vivo Characterization and Genomic Analysis at Single Cell Level
  • 08:59Conclusion

Özet

Otomatik Çeviri

该协议是从非目标背景细胞的混合物中恢复和制备稀有靶细胞, 用于单细胞水平的分子遗传表征。DNA 质量等于未处理的单个细胞, 并允许单细胞应用 (筛查和靶向分析)。

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