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Dissecação da Retina humana e RPE coroide para análise proteômica
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November 12, 2017
DOI:
1Barbara & Donald Jonas Stem Cell Laboratory, and Bernard & Shirlee Brown Glaucoma Laboratory, Department of Pathology & Cell Biology, Institute of Human Nutrition, College of Physicians and Surgeons,Columbia University, 2Edward S. Harkness Eye Institute,New York-Presbyterian Hospital, 3Omics Laboratory, Byers Eye Institute, Department of Ophthalmology,Stanford University, 4Medical Scientist Training Program,University of Iowa, 5Pediatri Anabilim Dalı,University of Iowa, 6Department of Neurology,University of Iowa, 7Department of Ophthalmology,Federal University of Sao Paulo (UNIFESP), 8Department of Ophthalmology,Federal University of EspÍrito Santo (UFES), 9Palo Alto Veterans Administration, Palo Alto, CA
Özet
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A retina humana é composta de regiões funcionalmente e molecularmente distintas, incluindo a fóvea, mácula e retina periférica. Aqui, descrevemos um método usando o soco de biópsias e remoção manual das camadas de tecido do olho humano para dissecar e coletar estas regiões distintas da retina para análise proteômica a jusante.
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Cite this Article
Cabral, T., Toral, M. A., Velez, G., DiCarlo, J. E., Gore, A. M., Mahajan, M., Tsang, S. H., Bassuk, A. G., Mahajan, V. B. Dissection of Human Retina and RPE-Choroid for Proteomic Analysis. J. Vis. Exp. (129), e56203, doi:10.3791/56203 (2017).
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