Dergi
/
İmmünoloji ve Enfeksiyon
/
Utilization of Capsules for Negative Staining of Viral Samples within Biocontainment
JoVE Journal
İmmünoloji ve Enfeksiyon
Bu içeriği görüntülemek için JoVE aboneliği gereklidir.  Oturum açın veya ücretsiz deneme sürümünü başlatın.
JoVE Journal İmmünoloji ve Enfeksiyon
Utilization of Capsules for Negative Staining of Viral Samples within Biocontainment

Utilization of Capsules for Negative Staining of Viral Samples within Biocontainment

9,955 Views

•

08:22 min

•

July 19, 2017

DOI:

10.3791/56122-v

DOI:
10.3791/56122-v

•

08:22 min
July 19, 2017

•

5 Views
, , , , , , , ,
1Pathology Division,United States Army Medical Research Institute of Infectious Diseases (USAMRIID), 2Diagnostic Systems Division,United States Army Medical Research Institute of Infectious Diseases (USAMRIID), 3Microscopy Innovations LLC

Özet

Automatically generated

This protocol provides instruction for negative staining virus samples which can easily be used in BSL-2, -3, or -4 laboratories. It includes the use of an innovative processing capsule, which protects the transmission electron microscopy grid and provides the user easier handling in the more turbulent environments within biocontainment.

Explore More Videos

VirusElectron MicroscopyNegative StainingBiocontainmentTEM GridsCapsulesGlutaraldehydeUranyl AcetatePotassium Phosphotungstic AcidOsmium TetroxideNanoparticles

Read Article

Cite this Article

Blancett, C. D., Monninger, M. K., Nguessan, C. A., Kuehl, K. A., Rossi, C. A., Olschner, S. P., Williams, P. L., Goodman, S. L., Sun, M. G. Utilization of Capsules for Negative Staining of Viral Samples within Biocontainment. J. Vis. Exp. (125), e56122, doi:10.3791/56122 (2017).

Download .ris file

Copy

Share Video

.

Copy