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使用CRISPR并发剂的小鼠小肠组织中多基因敲除的方案

JoVE Journal
Biyomühendislik

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JoVE Journal Biyomühendislik

A Protocol for Multiple Gene Knockout in Mouse Small Intestinal Organoids Using a CRISPR-concatemer

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使用CRISPR并发剂的小鼠小肠组织中多基因敲除的方案

17,567 Views

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11:53 min

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July 12, 2017

DOI:

10.3791/55916-v

DOI:

10.3791/55916-v

•

11:53 min

July 12, 2017

•

34 Views

Alessandra Merenda^1,2, Amanda Andersson-Rolf^1,2, Roxana C. Mustata¹, Taibo Li¹, Hyunki Kim³, Bon-Kyoung Koo^1,2

¹Wellcome Trust – Medical Research Council Stem Cell Institute,University of Cambridge, ²Genetik Bölümü,University of Cambridge, ³Patoloji Bölümü,Yonsei University College of Medicine

Özet

该协议描述了将多个单个引导RNA克隆到一个引导RNA并行载体中的步骤，其特别用于使用CRISPR / Cas9技术创建多基因敲除。肠组织中双重敲除的产生显示为该方法的可能应用。

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