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ODELAY：酵母増殖の多パラメータ定量化のための大規模法
JoVE Journal
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ODELAY: A Large-scale Method for Multi-parameter Quantification of Yeast Growth
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ODELAY：酵母増殖の多パラメータ定量化のための大規模法

7,921 Views

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11:19 min

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July 03, 2017

DOI:

10.3791/55879-v

DOI:
10.3791/55879-v

•

11:19 min
July 03, 2017

•

3 Views
, , ,
1Institute for Systems Biology, 2Center for Infectious Disease Research

Özet

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私たちは、個々の酵母細胞の成長表現型を、酵母（ODELAY）の生存アレイの1細胞倍加評価と称する経時的顕微鏡法を用いて固体培地上でコロニー中に増殖させる際に定量する方法を提示する。コロニーに成長する遺伝的に同一の細胞の集団の異種性を直接観察および定量することができる。

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Herricks, T., Mast, F. D., Li, S., Aitchison, J. D. ODELAY: A Large-scale Method for Multi-parameter Quantification of Yeast Growth. J. Vis. Exp. (125), e55879, doi:10.3791/55879 (2017).

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