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ODELAY：用于酵母生长多参数定量的大规模方法
JoVE Journal
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JoVE Journal Biyoloji
ODELAY: A Large-scale Method for Multi-parameter Quantification of Yeast Growth
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ODELAY：用于酵母生长多参数定量的大规模方法

7,921 Views

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11:19 min

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July 03, 2017

DOI:

10.3791/55879-v

DOI:
10.3791/55879-v

•

11:19 min
July 03, 2017

•

3 Views
, , ,
1Institute for Systems Biology, 2Center for Infectious Disease Research

Özet

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我们提出了一种定量单个酵母细胞的生长表型的方法，它们使用称为酵母生活阵列的单细胞倍增评估（ODELAY）的延时显微镜在固体培养基上生长成菌落。可以直接观察和量化生长到菌落中的遗传相同细胞的群体异质性。

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Herricks, T., Mast, F. D., Li, S., Aitchison, J. D. ODELAY: A Large-scale Method for Multi-parameter Quantification of Yeast Growth. J. Vis. Exp. (125), e55879, doi:10.3791/55879 (2017).

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