Dergi
/
İmmünoloji ve Enfeksiyon
/
Méthodes in vitro pour la comparaison de liaison cible et CDC induction entre les anticorps thérapeutiques: Applications dans l' analyse biosimilarité
JoVE Journal
İmmünoloji ve Enfeksiyon
Bu içeriği görüntülemek için JoVE aboneliği gereklidir.  Oturum açın veya ücretsiz deneme sürümünü başlatın.
JoVE Journal İmmünoloji ve Enfeksiyon
In Vitro Methods for Comparing Target Binding and CDC Induction Between Therapeutic Antibodies: Applications in Biosimilarity Analysis
Please note that all translations are automatically generated. Click here for the English version.

Méthodes in vitro pour la comparaison de liaison cible et CDC induction entre les anticorps thérapeutiques: Applications dans l' analyse biosimilarité

17,565 Views

•

07:25 min

•

May 04, 2017

DOI:

10.3791/55542-v

DOI:
10.3791/55542-v

•

07:25 min
May 04, 2017

•

3 Views
, , , ,
1Unit for Development and Research in Bioprocesses Unit (UDIBI), National School of Biological Sciences,National Polytechnic Institute (IPN), University of Mexico (UNAM), 2School of Chemistry,National Autonomous University of Mexico (UNAM), 3Graduate Program in Chemical Sciences,National Autonomous University of Mexico (UNAM), 4Unit for Development Research and Medical Innovation in Biotechnology (UDIMEB), National School of Biological Sciences,National Polytechnic Institute (IPN), 5Department of Immunology, National Scool of Biological Sciences,National Polytechnic Institute (IPN), 6Department of Pharmacology and Unit of Translational Biomedicine (CMN 20 de noviembre), School of Medicine,National Autonomous University of Mexico (UNAM)

Özet

Automatically generated

Ce protocole décrit la comparaison in vitro de deux caractéristiques fonctionnelles principales du rituximab: liant cible et l'induction de cytotoxicité dépendante du complément (CDC). Les méthodes ont été utilisées pour une comparaison côte-à-côte entre le rituximab de référence et un rituximab biosimilaire. Ces tests peuvent être utilisés pendant le développement biosimilaire ou comme un contrôle de qualité dans leur production.

Explore More Videos

Therapeutic AntibodiesBiosimilarity AnalysisTarget BindingComplement-dependent Cytotoxicity (CDC) InductionIn Vitro MethodsRituximabDaudi CellsReference ProductBiosimilarQuality Control

Read Article

Cite this Article

Salinas-Jazmín, N., González-González, E., Vásquez-Bochm, L. X., Pérez-Tapia, S. M., Velasco-Velázquez, M. A. In Vitro Methods for Comparing Target Binding and CDC Induction Between Therapeutic Antibodies: Applications in Biosimilarity Analysis. J. Vis. Exp. (123), e55542, doi:10.3791/55542 (2017).

Download .ris file

Copy

Share Video

.

Copy