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使用电刺激人干细胞衍生的心肌细胞的成熟中Biowires
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Gelişim Biyolojisi
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JoVE Journal Gelişim Biyolojisi
Maturation of Human Stem Cell-derived Cardiomyocytes in Biowires Using Electrical Stimulation
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使用电刺激人干细胞衍生的心肌细胞的成熟中Biowires

DOI:
10.3791/55373-v

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10:11 min

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May 06, 2017

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,
1Toronto General Research Institute,University Health Network, 2Institute of Biomaterials and Biomedical Engineering,University of Toronto, 3Heart & Stroke/Richard Lewar Centre of Excellence,University of Toronto, 4Laboratory of Medicine and Pathobiology,University of Toronto

Bölümler

  • 00:05Başlık
  • 01:01Fabrication of Biowire Templates
  • 02:22Fabricating the Electrical Simulation Chambers
  • 03:55Gel Preparation and Seeding of Cardiomyocytes into the Template
  • 05:41Culturing Cardiomyocytes in the Template with Electrical Stimulation (Biowire)
  • 07:22Results: Biowire Cardiomyocyte Physiology
  • 09:16Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

心脏biowire平台是使用三维细胞培养与电刺激结合成熟人胚胎和诱导的多能干细胞衍生的心肌细胞（HPSC-CM）的体外方法。这份手稿介绍了心脏biowire平台的详细设置。

Etiketler

Stem CellCardiomyocyteMaturationElectrical StimulationBiowireDrug ScreeningPDMSSuturePolycarbonateCarbon RodPlatinum Wire

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