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一种内在的弥漫性脑胶质瘤脑桥快速验尸细胞培养协议(DIPG)
JoVE Journal
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JoVE Journal Kanser Araştırmaları
A Protocol for Rapid Post-mortem Cell Culture of Diffuse Intrinsic Pontine Glioma (DIPG)

一种内在的弥漫性脑胶质瘤脑桥快速验尸细胞培养协议(DIPG)

DOI:

08:46 min

March 07, 2017

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Bölümler

  • 00:05Başlık
  • 01:12Mechanical Dissociation
  • 03:25Enzymatic Dissociation
  • 04:50Sucrose Gradient Centrifugation
  • 05:49ACK Red Blood Cell Lysis and Initial Culture Maintenance
  • 06:50Results: Images of Primary Cultures and Early-passage Cells
  • 07:41Conclusion

Özet

Otomatik Çeviri

本协议描述为事后漫内在脑桥脑胶质瘤样品进行建立以病人来源的细胞培养模型或肿瘤微环境和细胞的直接表征的快速处理的方法。

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