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האדם תאים דנדריטים ריאות: הפריסה המרחבית וזיהוי פנוטיפי ב Endobronchial ביופסיות שימוש אימונוהיסטוכימיה ו cytometry זרימה
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İmmünoloji ve Enfeksiyon
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JoVE Journal İmmünoloji ve Enfeksiyon
Human Lung Dendritic Cells: Spatial Distribution and Phenotypic Identification in Endobronchial Biopsies Using Immunohistochemistry and Flow Cytometry
Please note that all translations are automatically generated. Click here for the English version.

האדם תאים דנדריטים ריאות: הפריסה המרחבית וזיהוי פנוטיפי ב Endobronchial ביופסיות שימוש אימונוהיסטוכימיה ו cytometry זרימה

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11:02 min

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January 20, 2017

DOI:

10.3791/55222-v

DOI:
10.3791/55222-v

•

11:02 min
January 20, 2017

•

1 Views
, , , , , ,
1Immunology and Allergy Unit, Department of Medicine Solna,Karolinska Institutet, 2Division of Medicine, Department of Public Health and Clinical Medicine,Umeå University, 3Divison of Infectious Diseases, Department of Clinical Microbiology,Umeå University

Özet

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Lung-resident immune cells, including dendritic cells (DCs) in humans, are critical for defense against inhaled pathogens and allergens. However, due to the scarcity of human lung tissue, studies are limited. This work presents protocols to process human mucosal endobronchial biopsies for studying lung DCs using immunohistochemistry and flow cytometry.

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Lung Dendritic CellsEndobronchial BiopsiesImmunohistochemistryFlow CytometryMucosal ImmunologyCOPDSarcoidosisLung CancerSpatial DistributionPhenotypic Identification

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Baharom, F., Rankin, G., Scholz, S., Pourazar, J., Ahlm, C., Blomberg, A., Smed-Sörensen, A. Human Lung Dendritic Cells: Spatial Distribution and Phenotypic Identification in Endobronchial Biopsies Using Immunohistochemistry and Flow Cytometry. J. Vis. Exp. (119), e55222, doi:10.3791/55222 (2017).

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