Dergi
/
Biyokimya
/
使用中性毛细管单克隆抗体亚型的毛细管电泳分离
JoVE Journal
Biyokimya
Author Produced
Bu içeriği görüntülemek için JoVE aboneliği gereklidir.  Oturum açın veya ücretsiz deneme sürümünü başlatın.
JoVE Journal Biyokimya
Capillary Electrophoresis Separation of Monoclonal Antibody Isoforms Using a Neutral Capillary
Please note that all translations are automatically generated. Click here for the English version.

使用中性毛细管单克隆抗体亚型的毛细管电泳分离

DOI:
10.3791/55082-v

•

14:53 min

•

January 16, 2017

•

, , , ,
1Unidad de Investigación y Desarrollo, Probiomed S.A. de C.V.

Bölümler

  • 00:00Başlık
  • 00:56Preparation of Background Electrolyte (BGE) Solution
  • 02:18Sample Preparation
  • 03:45CE System Cleaning
  • 05:46Cartridge Assembly
  • 09:41Preparation of Buffer Trays
  • 10:44CE System Components Assembly
  • 12:10Instrument Methods Set-up
  • 13:21Data Acquisition and Processing
  • 14:11Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

Here, we present a comprehensive capillary zone electrophoresis protocol for the assessment of intrinsic physicochemical heterogeneity of monoclonal antibodies as a quality attribute.

Etiketler

Capillary ElectrophoresisMonoclonal AntibodyIsoformsHPMCBuffer SolutionSample PreparationCE Instrument

Article

Embed

ÇALMA LİSTESİNE EKLE

Usage Statistics

İlgili Videolar

鞘毛细管电泳 - 质谱联用生物样品的代谢谱

使用非变性聚丙烯酰胺凝胶电泳人的MxA多亚基蛋白复合物的表征

实验室规模的生产和治疗性抗体的纯化

高亲和力抗体 - 抗原的结合动力学的测定，使用四个生物传感器平台

毛细管电聚焦法对蛋白质及其亚型的高灵敏度和定量检测

毛细管电泳与亲和毛细管电泳对金属离子 AtHIRD11 固有紊乱与结合的分析

原位近接结扎法检测蛋白异构体的 Heterodimerization

混合光/蓝色原生电泳在线粒体呼吸链超综合体分离与分析中的作用

用噻唑橙代替溴代或替代染料进行 DNA 电泳

用利甘德分析（DRACALA）的微分径向毛细管作用识别小配体的结合蛋白

Read Article