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细胞形态改变与细胞运动的影像<em>果蝇</em>原肠胚形成使用DE-cadherin的记者转基因果蝇
JoVE Journal
Gelişim Biyolojisi
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JoVE Journal Gelişim Biyolojisi
Imaging of Cell Shape Alteration and Cell Movement in Drosophila Gastrulation Using DE-cadherin Reporter Transgenic Flies
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细胞形态改变与细胞运动的影像<em>果蝇</em>原肠胚形成使用DE-cadherin的记者转基因果蝇

DOI:
10.3791/54764-v

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04:42 min

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December 29, 2016

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, , , ,
1Biotechnology and Genetic Engineering Department,Jahangirnagar University, 2JT Biohistory Research Hall, 3Division of Cell Regeneration and Transplantation, School of Medicine,Nihon University, 4Graduate School of Life and Medical Sciences,Doshisha University, 5Institute of Genetics and Animal Breeding of the Polish Academy of Sciences

Bölümler

  • 00:05Başlık
  • 00:39Apple Plate Preparation
  • 01:24Embryo Preparation
  • 02:30Live Imaging
  • 03:43Sonuçlar
  • 04:13Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

Herein we describe a procedure to capture live images of Drosophila gastrulation. This has enabled us to better understand the apical constriction involved in early development and further analyze mechanisms governing cellular movements during tissue structure modification.

Etiketler

Cell ShapeCell MovementDrosophila GastrulationDE-cadherinReporter Transgenic FliesCellular RearrangementApical ConstrictionEmbryonic DevelopmentImagingMicroscopySample PreparationEmbryo CollectionDechorionationOrientationMounting

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