Imaging of Cell Shape Alteration and Cell Movement in Drosophila Gastrulation Using DE-cadherin Reporter Transgenic Flies

M. Rezaul Karim; Tomohiro Haruta; Taro Matsumoto; Hiroki Oda; Hiroaki Taniguchi

doi:10.3791/54764

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Imaging di cellule di figura alterazione e la cella in Movimento Drosophila gastrulation Utilizzando DE-caderina Reporter mosche transgeniche

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JoVE Journal Gelişim Biyolojisi

Imaging of Cell Shape Alteration and Cell Movement in Drosophila Gastrulation Using DE-cadherin Reporter Transgenic Flies

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Imaging di cellule di figura alterazione e la cella in Movimento Drosophila gastrulation Utilizzando DE-caderina Reporter mosche transgeniche

DOI:

10.3791/54764-v

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04:42 min

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December 29, 2016

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M. Rezaul Karim, Tomohiro Haruta, Taro Matsumoto, Hiroki Oda, Hiroaki Taniguchi^4,5

¹Biotechnology and Genetic Engineering Department,Jahangirnagar University, ²JT Biohistory Research Hall, ³Division of Cell Regeneration and Transplantation, School of Medicine,Nihon University, ⁴Graduate School of Life and Medical Sciences,Doshisha University, ⁵Institute of Genetics and Animal Breeding of the Polish Academy of Sciences

Bölümler

00:05Başlık
00:39Apple Plate Preparation
01:24Embryo Preparation
02:30Live Imaging
03:43Sonuçlar
04:13Conclusion

Özet

Otomatik Çeviri

Herein we describe a procedure to capture live images of Drosophila gastrulation. This has enabled us to better understand the apical constriction involved in early development and further analyze mechanisms governing cellular movements during tissue structure modification.

Etiketler

Cell Shape Cell Movement Drosophila Gastrulation DE-cadherin Reporter Transgenic Flies Cellular Rearrangement Apical Constriction Embryonic Development Imaging Microscopy Sample Preparation Embryo Collection Dechorionation Orientation Mounting

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Imaging di cellule di figura alterazione e la cella in Movimento<em> Drosophila</em> gastrulation Utilizzando DE-caderina Reporter mosche transgeniche

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