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Automated Analysis of C. elegans Swim Behavior Using CeleST Software
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JoVE Journal Nörobilim
Automated Analysis of C. elegans Swim Behavior Using CeleST Software

Automated Analysis of C. elegans Swim Behavior Using CeleST Software

DOI:
10.3791/54359-v

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08:47 min

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December 07, 2016

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, , , ,
1Department of Molecular Biology and Biochemistry,Rutgers University, 2Computing Research and Education Building (CoRE),Rutgers University

Bölümler

  • 00:05Başlık
  • 00:48Setup and Recording of Swim Analysis
  • 03:06Post-video Processing
  • 04:17Computer Measurements
  • 05:49Analysis of Swimming Behavior
  • 07:23Results: Nematode Swimming Behavior Changes with Age
  • 08:09Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

An efficient and simple methodology for computer-based analysis of nematode swimming behavior in liquid is described. The method requires little to no investment for C. elegans laboratories. The hardware used is standard, and the computer software for the behavioral analysis (CeleST) is an open source one.

Etiketler

C. ElegansSwim BehaviorCeleST SoftwareComputer VisionAutomated AnalysisLocomotive CapacitiesGenetic MutationsPharmacological InterventionsVideo RecordingMicroscopyDark Field IlluminationM9 BufferAdult NematodesImage FramesMATLAB

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