This protocol provides step-by-step guidelines for setting up competitive mouse bone marrow transplant experiments to study hematopoietic stem/progenitor cell function without prior purification of stem cells by cell sorting.
Kwarteng, E. O., Heinonen, K. M. Competitive Transplants to Evaluate Hematopoietic Stem Cell Fitness. J. Vis. Exp. (114), e54345, doi:10.3791/54345 (2016).