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소설 불용성 발색 기질 분석 키트를 사용하여 탄수화물 분해 효소의 높은 처리량 검열
JoVE Journal
Biyokimya
This content is Free Access.
JoVE Journal Biyokimya
High-throughput Screening of Carbohydrate-degrading Enzymes Using Novel Insoluble Chromogenic Substrate Assay Kits

소설 불용성 발색 기질 분석 키트를 사용하여 탄수화물 분해 효소의 높은 처리량 검열

DOI:

06:51 min

September 20, 2016

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Bölümler

  • 00:05Başlık
  • 00:40Chromogenic Assay with CPH Substrates, Detection, and Quantification
  • 02:41Chromogenic Assay with Insoluble Chromogenic Biomass Substrates, Detection, and Quantification
  • 04:15Results: Enzymatic Degradation of Chromogenic Polymer Hydrogel Substrates
  • 06:02Conclusion

Özet

Otomatik Çeviri

A high-throughput assay for enzyme screening is described. This multiplexed ready-to-use assay kit comprises of pre-chosen Chromogenic Polymer Hydrogel (CPH) substrates and complex Insoluble Chromogenic Biomass (ICB) substrates. Target enzymes are polysaccharide degrading endo-enzymes and proteases.

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