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Combinando Duplo Fluorescência<em> In Situ</em> A hibridação com imunomarcação para a detecção da expressão de três genes em secções do cérebro do rato
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March 26, 2016
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Localizing gene expression to specific cell types can be challenging due to the lack of specific antibodies. Here we describe a protocol for simultaneous triple detection of gene expression by combining double fluorescence RNA in situ hybridization with immunostaining.
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Double Fluorescence In Situ HybridizationImmunolabellingGene ExpressionMouse Brain SectionsCell Type LocalizationRNA ProbesFITCDIGTyramideAlkaline PhosphataseFast RedImmunostainingPlasmid DNARNA Polymerase PromotersCDNARestriction EnzymeLinearizationPurificationPhenol-chloroform ExtractionEthanol Precipitation
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Jolly, S., Fudge, A., Pringle, N., Richardson, W. D., Li, H. Combining Double Fluorescence In Situ Hybridization with Immunolabelling for Detection of the Expression of Three Genes in Mouse Brain Sections. J. Vis. Exp. (109), e53976, doi:10.3791/53976 (2016).
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