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Utilisation de feuilles Lumière Fluorescence Microscopy à l'image du développement Zebrafish Eye
JoVE Journal
Gelişim Biyolojisi
This content is Free Access.
JoVE Journal Gelişim Biyolojisi
Using Light Sheet Fluorescence Microscopy to Image Zebrafish Eye Development

Utilisation de feuilles Lumière Fluorescence Microscopy à l'image du développement Zebrafish Eye

DOI:

13:01 min

April 10, 2016

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Bölümler

  • 00:05Başlık
  • 01:34Preparing the Microscope
  • 02:42Preparing the Sample
  • 05:46Image Processing
  • 10:33Results: Light Sheet Fluorescent Microscopy (LSFM) Images
  • 11:58Conclusion

Özet

Otomatik Çeviri

Light sheet fluorescence microscopy is an excellent tool for imaging embryonic development. It allows recording of long time-lapse movies of live embryos in near physiological conditions. We demonstrate its application for imaging zebrafish eye development across wide spatio-temporal scales and present a pipeline for fusion and deconvolution of multiview datasets.

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