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子供からプライマリ鼻上皮細胞を培養し、人工多能性幹細胞に再プログラム
JoVE Journal
Gelişim Biyolojisi
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JoVE Journal Gelişim Biyolojisi
Cultivate Primary Nasal Epithelial Cells from Children and Reprogram into Induced Pluripotent Stem Cells
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子供からプライマリ鼻上皮細胞を培養し、人工多能性幹細胞に再プログラム

DOI:
10.3791/53814-v

•

12:08 min

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March 10, 2016

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, , , ,
1Pyrosequencing Core,Cincinnati Children’s Hospital, 2Division of Developmental Biology,Cincinnati Children’s Hospital, 3Division of Pulmonary Medicine,Seattle Children’s Hospital, 4Division of Asthma Research,Cincinnati Children’s Hospital

Bölümler

  • 00:05Başlık
  • 01:13Sampling the Nasal Mucosa
  • 02:50Counting and Spinning Cells
  • 04:20Seeding Cells
  • 05:06Reprogramming to iPSCs
  • 09:11Results: Generation of iPSCs from Nasal Epithelial Cells
  • 10:47Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

This publication demonstrates methods for successful sampling and culture of nasal epithelial mucosa from children, and reprogramming these cells to induced Pluripotent Stem Cells (iPSCs).

Etiketler

Nasal Epithelial CellsInduced Pluripotent Stem CellsIPSCsAerated DiseaseAsthmaEpigenetic VariationAir PollutionNasal SamplingReprogrammingBEGMCytology BrushLive/dead Cell Stain

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