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Cultiver des cellules primaires épithéliales nasales des enfants et des Reprogrammer dans les cellules souches pluripotentes induites
JoVE Journal
Gelişim Biyolojisi
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JoVE Journal Gelişim Biyolojisi
Cultivate Primary Nasal Epithelial Cells from Children and Reprogram into Induced Pluripotent Stem Cells

Cultiver des cellules primaires épithéliales nasales des enfants et des Reprogrammer dans les cellules souches pluripotentes induites

DOI:

12:08 min

March 10, 2016

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Bölümler

  • 00:05Başlık
  • 01:13Sampling the Nasal Mucosa
  • 02:50Counting and Spinning Cells
  • 04:20Seeding Cells
  • 05:06Reprogramming to iPSCs
  • 09:11Results: Generation of iPSCs from Nasal Epithelial Cells
  • 10:47Conclusion

Özet

Otomatik Çeviri

This publication demonstrates methods for successful sampling and culture of nasal epithelial mucosa from children, and reprogramming these cells to induced Pluripotent Stem Cells (iPSCs).

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