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Epikardiale Outgrowth Kulturtest und<em> Ex Vivo</em> Beurteilung von Epikardiale abgeleiteten Zellmigration
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Epikardiale Outgrowth Kulturtest und<em> Ex Vivo</em> Beurteilung von Epikardiale abgeleiteten Zellmigration
Bölümler
- 00:05Başlık
- 00:49Epicardial Outgrowth Culture and Genetic Ablation
- 03:31Epicardial Labeling and Ex Vivo Migration Culture
- 05:17Results: Representative Epicardial Outgrowth Purity
- 06:23Representative Ex Vivo Migration Assay
- 06:56Conclusion
Here, we describe methods for isolating primary mouse epicardial cells by an outgrowth culture assay and assessing the functional migration of epicardial-derived cells (EPDC) using an ex vivo heart culture system. These protocols are suitable for identifying genetic and chemical modulators of epicardial epithelial-to-mesenchymal transition (EMT) and motility.
