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A Quick and Efficient Method for the Purification of Endoderm Cells Generated from Human Embryonic Stem Cells
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Gelişim Biyolojisi
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JoVE Journal Gelişim Biyolojisi
A Quick and Efficient Method for the Purification of Endoderm Cells Generated from Human Embryonic Stem Cells

A Quick and Efficient Method for the Purification of Endoderm Cells Generated from Human Embryonic Stem Cells

DOI:
10.3791/53655-v

•

08:34 min

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March 03, 2016

•

, ,
1Institute of Clinical Biochemistry,Hannover Medical School

Bölümler

  • 00:05Başlık
  • 00:34Human ES Cell Differentiation toward the Definitive Endoderm
  • 02:57CXCR4+ Definitive Endoderm (DE) Cell Immunostaining for Flow Cytometric Analysis
  • 04:55CXCR4+ Cell Magnetic Bead Isolation
  • 06:34Results: Representative Changes in Gene/Protein Expression after Human ESC into DE Differentiation
  • 07:59Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

Here, we describe a method for the purification of differentiated human embryonic stem cells that are committed towards the definitive endoderm for the improvement of downstream applications and further differentiations.

Etiketler

Endoderm CellsHuman Embryonic Stem CellsPurificationDifferentiationCell CultureBasement Membrane MatrixROCK InhibitorPrimitive Streak InductionEndoderm InductionSingle-cell Suspension

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