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Air-sampled Filter Analysis for Endotoxins and DNA Content
JoVE Journal
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JoVE Journal Çevre
Air-sampled Filter Analysis for Endotoxins and DNA Content

Air-sampled Filter Analysis for Endotoxins and DNA Content

DOI:
10.3791/53444-v

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09:16 min

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March 07, 2016

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, , ,
1Department of Earth and Planetary Sciences,Weizmann Institute of Science, 2Multiphase Chemistry Department,Max Planck Institute

Bölümler

  • 00:05Başlık
  • 00:34Air Filter Preparation and Sampling
  • 01:38Extraction of Endotoxin from Filter Samples
  • 02:51Limulus Amebocyte Lysate Endotoxin Test
  • 04:30DNA Extraction from Experimental and Spiked Filters
  • 06:02Quantitative PCR Analysis
  • 07:48Results: Centrifugation Speed and Filter Load Influences the Measured Endotoxin Concentrations in Samples
  • 08:35Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

Two complementary analyses of atmospheric biological particles from air sampled filters are described herein: the extraction and detection of endotoxin, and of DNA.

Etiketler

Air SamplingFilter AnalysisEndotoxinsDNA ContentBiological Aerosol ResearchHigh Volume SamplingQuartz Fiber FiltersOrganic And Biological Compound SamplingBaked FiltersHigh Volume SamplerBiosafety CabinetEndotoxin Spiked FiltersPyrogen Free TubesMicrocentrifugeEndotoxin TestMicroplate ReaderKinetic Reaction

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