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아데노 바이러스에 감염된 정상적인 인간의 세포에서 바이러스 복제 구획 풍부한 하위 핵 분수의 분리
Bölümler
- 00:05Başlık
- 00:57Preparation of the Subnuclear Fraction Enriched in Adenoviral Replication Compartments
- 05:54Immunofluorescence Visualization of RCf
- 08:00Results: Presence of Adenoviral Components in the Replication Compartment Fraction from Infected Human Foreskin Fibroblasts
- 08:49Conclusion
We provide a novel strategy to isolate viral replication compartments (RC) from adenovirus (Ad)-infected human cells. This approach represents a cell-free system that can help to elucidate the molecular mechanisms regulating viral genome replication and expression as well as regulation of viral-host interactions established at the RC.
