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由腺病毒感染正常细胞分离病毒复制车厢富集亚核分数
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JoVE Journal İmmünoloji ve Enfeksiyon
Isolation of Viral Replication Compartment-enriched Sub-nuclear Fractions from Adenovirus-infected Normal Human Cells
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由腺病毒感染正常细胞分离病毒复制车厢富集亚核分数

8,633 Views

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10:22 min

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November 12, 2015

DOI:

10.3791/53296-v

DOI:
10.3791/53296-v

•

10:22 min
November 12, 2015

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1 Views
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1Centro de Investigación en Dinámica Celular, Instituto de Investigación en Ciencias Básicas y Aplicadas,Universidad Autónoma del Estado de Morelos, 2Instituto de Biotecnologìa,Universidad Nacional Autónoma de México

Özet

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We provide a novel strategy to isolate viral replication compartments (RC) from adenovirus (Ad)-infected human cells. This approach represents a cell-free system that can help to elucidate the molecular mechanisms regulating viral genome replication and expression as well as regulation of viral-host interactions established at the RC.

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Hidalgo, P., Gonzalez, R. A. Isolation of Viral Replication Compartment-enriched Sub-nuclear Fractions from Adenovirus-infected Normal Human Cells. J. Vis. Exp. (105), e53296, doi:10.3791/53296 (2015).

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