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경쟁 분석법을 이용는 GTPase - 결합 단백질의 친화 비교
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October 08, 2015
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This protocol compares the relative affinities of binding partners for Rho-family GTPases, including Rac1. In vivo, Rac1-binding proteins compete for a single binding interface, the conformation of which is dictated by a bound nucleotide. The nucleotide is both important and difficult to control experimentally, due to the high hydrolysis rate.
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Williamson, R. C., Bass, M. D. Comparing the Affinity of GTPase-binding Proteins using Competition Assays. J. Vis. Exp. (104), e53254, doi:10.3791/53254 (2015).
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