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Métodos para investigar o papel de Regulamentação dos Pequenos RNAs e Ribosomal Ocupação de<em> Plasmodium falciparum</em
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JoVE Journal İmmünoloji ve Enfeksiyon
Methods to Investigate the Regulatory Role of Small RNAs and Ribosomal Occupancy of Plasmodium falciparum

Métodos para investigar o papel de Regulamentação dos Pequenos RNAs e Ribosomal Ocupação de<em> Plasmodium falciparum</em

8,744 Views

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10:22 min

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December 04, 2015

DOI:

10.3791/53214-v

DOI:
10.3791/53214-v

•

10:22 min
December 04, 2015

•

1 Views
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1Department of Molecular Genetics and Microbiology,Duke University School of Medicine, 2Center for Genomic and Computational Biology,Duke University School of Medicine, 3Department of Cell Biology,Duke University School of Medicine

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Human microRNAs translocate from host erythrocytes to Plasmodium falciparum parasites. Here, the techniques used to transfect synthetic microRNAs into host erythrocytes and isolate all RNAs from P. falciparum are described. In addition, this paper will detail a method of polysome isolation in P. falciparum to determine the ribosomal occupancy and translational potential of parasite transcripts.

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LaMonte, G., Walzer, K. A., Lacsina, J., Nicchitta, C., Chi, J. Methods to Investigate the Regulatory Role of Small RNAs and Ribosomal Occupancy of Plasmodium falciparum. J. Vis. Exp. (106), e53214, doi:10.3791/53214 (2015).

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