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L'étude de la Stœchiométrie Epidermal Growth Factor Receptor dans des cellules intactes en utilisant la microscopie corrélative
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L'étude de la Stœchiométrie Epidermal Growth Factor Receptor dans des cellules intactes en utilisant la microscopie corrélative
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September 11, 2015
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This protocol describes the labeling of epidermal growth factor receptor (EGFR) on COS7 fibroblast cells, and subsequent correlative light- and electron microscopy of whole cells in hydrated state. The label contained fluorescent quantum dots. The protocol can be used to study the stoichiometry of EGFR at the single molecule level.
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Peckys, D. B., de Jonge, N. Studying the Stoichiometry of Epidermal Growth Factor Receptor in Intact Cells using Correlative Microscopy. J. Vis. Exp. (103), e53186, doi:10.3791/53186 (2015).
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