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脑组织中的双管和同心微电极用于测量细胞外离子的信号
JoVE Journal
Nörobilim
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JoVE Journal Nörobilim
Double-barreled and Concentric Microelectrodes for Measurement of Extracellular Ion Signals in Brain Tissue
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脑组织中的双管和同心微电极用于测量细胞外离子的信号

13,152 Views

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11:08 min

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September 05, 2015

DOI:

10.3791/53058-v

DOI:
10.3791/53058-v

•

11:08 min
September 05, 2015

•

6 Views
, , , ,
1Institute of Neurobiology,Heinrich Heine University Düsseldorf, 2Departments of Physiology and Neuroscience,New York University School of Medicine

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We demonstrate the fabrication, calibration and properties of two types of ion-selective microelectrodes (double-barreled and concentric) for measurement of ion concentrations in brain tissue. These are then used in the mouse hippocampal slice preparation to show that excitatory activity changes both extracellular potassium and sodium concentrations.

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Double-barreled MicroelectrodesConcentric MicroelectrodesExtracellular Ion SignalsPotassiumSodiumHippocampusAcute Brain SliceIon-selective Electrodes

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Haack, N., Durry, S., Kafitz, K. W., Chesler, M., Rose, C. R. Double-barreled and Concentric Microelectrodes for Measurement of Extracellular Ion Signals in Brain Tissue. J. Vis. Exp. (103), e53058, doi:10.3791/53058 (2015).

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