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Kupffer isolamento celular para Nanoparticle Toxicity Testing
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August 18, 2015
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Liver macrophages, named Kupffer cells, are responsible for the capture of circulating nanoparticles. We describe here a method, of high cell purity and yield, for Kupffer cell isolation. The modified LDH assay is used here to measure the toxicity induced by carbon nanotubes in Kupffer cells.
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Bourgognon, M., Klippstein, R., Al-Jamal, K. T. Kupffer Cell Isolation for Nanoparticle Toxicity Testing. J. Vis. Exp. (102), e52989, doi:10.3791/52989 (2015).
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