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Visualization of Chondrocyte Intercalation and Directional Proliferation via Zebrabow Clonal Cell Analysis in the Embryonic Meckel’s Cartilage
Bu içeriği görüntülemek için JoVE aboneliği gereklidir. Oturum açın veya ücretsiz deneme sürümünü başlatın.
Visualization of Chondrocyte Intercalation and Directional Proliferation via Zebrabow Clonal Cell Analysis in the Embryonic Meckel’s Cartilage
Bölümler
- 00:05Başlık
- 01:21Embryo Collection, Tamoxifen Treatment, and Embryo Selection
- 02:46Mounting Embryos in Methylcellulose and Fluorescence Microscopy
- 04:33Results: Chondrocyte Intercalation and Directional Proliferation in ubi:Zebrabow-M;sox10:ERT2-Cre Transgenic Zebrafish
- 06:15Conclusion
Cell organization of craniofacial bones has long been hypothesized but never directly visualized. Multi-spectral cell labeling and in vivo live imaging allows visualization of dynamic cell behavior in zebrafish lower jaw. Here, we detail the protocol to manipulate Zebrabow transgenic fish and directly observe cell intercalation and morphological changes of chondrocytes in the Meckel’s cartilage.
