We describe a protocol for hybrid imaging, combining fluorescence-mediated tomography (FMT) with micro computed tomography (µCT). After fusion and reconstruction, we perform interactive organ segmentation to extract quantitative measurements of the fluorescence distribution.
Gremse, F., Doleschel, D., Zafarnia, S., Babler, A., Jahnen-Dechent, W., Lammers, T., Lederle, W., Kiessling, F. Hybrid µCT-FMT imaging and image analysis. J. Vis. Exp. (100), e52770, doi:10.3791/52770 (2015).