This content is Free Access.
로봇 액체 처리 시스템을 사용하여 확장 가능한 96 웰 IPSC 문화 기반 플레이트 및 생산
31,279 Views
•
08:00 min
•
May 14, 2015
DOI:
Özet
Automatically generated
The protocols described allow laboratories to perform scalable, adherent stem cell culture in high throughput with minimal labor, experience and equipment investment cost using a programmable liquid handling robot and 96-well plates. iPSCs passaged more than 20 times on this system maintained pluripotency, normal karyotypes and differentiated into cardiomyocytes.
Read Article
Cite this Article
Conway, M. K., Gerger, M. J., Balay, E. E., O'Connell, R., Hanson, S., Daily, N. J., Wakatsuki, T. Scalable 96-well Plate Based iPSC Culture and Production Using a Robotic Liquid Handling System. J. Vis. Exp. (99), e52755, doi:10.3791/52755 (2015).
Copy