Dergi
/
/
Schaalbare 96 putjes plaat Based iPSC Culture and Production Met een Robotic Liquid Handling System
JoVE Journal
Gelişim Biyolojisi
This content is Free Access.
JoVE Journal Gelişim Biyolojisi
Scalable 96-well Plate Based iPSC Culture and Production Using a Robotic Liquid Handling System

Schaalbare 96 putjes plaat Based iPSC Culture and Production Met een Robotic Liquid Handling System

DOI:

08:00 min

May 14, 2015

, , , , , ,

Bölümler

  • 00:05Başlık
  • 01:54Feeding 96-well Plate Stem Cell Colonies
  • 03:26Passaging 96-well Plate Stem Cell Colonies
  • 06:03Results: Representative Plate Based Robotic Stem Cell Culture
  • 07:36Conclusion

Özet

Otomatik Çeviri

The protocols described allow laboratories to perform scalable, adherent stem cell culture in high throughput with minimal labor, experience and equipment investment cost using a programmable liquid handling robot and 96-well plates. iPSCs passaged more than 20 times on this system maintained pluripotency, normal karyotypes and differentiated into cardiomyocytes.

İlgili Videolar

Read Article