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수질 흉선 상피 세포의 증식, 분화 및 무차별 유전자 발현을 지원하는 3D의 Organotypic 공동 문화 모델
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Gelişim Biyolojisi
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JoVE Journal Gelişim Biyolojisi
3D Organotypic Co-culture Model Supporting Medullary Thymic Epithelial Cell Proliferation, Differentiation and Promiscuous Gene Expression
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수질 흉선 상피 세포의 증식, 분화 및 무차별 유전자 발현을 지원하는 3D의 Organotypic 공동 문화 모델

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06:47 min

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July 30, 2015

DOI:

10.3791/52614-v

DOI:
10.3791/52614-v

•

06:47 min
July 30, 2015

•

4 Views
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1Division of Developmental Immunology,German Cancer Research Center (DKFZ), 2Genetics of Skin Carcinogenesis,German Cancer Research Center (DKFZ)

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Studying medullary thymic epithelial cells in vitro has been largely unsuccessful, as current 2D culture systems do not mimic the in vivo scenario. The 3D culture system described herein - a modified skin organotypic culture model - has proven superior in recapitulating mTEC proliferation, differentiation and maintenance of promiscuous gene expression.

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Pinto, S., Stark, H., Martin, I., Boukamp, P., Kyewski, B. 3D Organotypic Co-culture Model Supporting Medullary Thymic Epithelial Cell Proliferation, Differentiation and Promiscuous Gene Expression. J. Vis. Exp. (101), e52614, doi:10.3791/52614 (2015).

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