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Imaging Ca2+ Dynamics in Cone Photoreceptor Axon Terminals of the Mouse Retina
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May 06, 2015
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1Institute for Ophthalmic Research,University of Tübingen, 2Graduate School of Cellular & Molecular Neuroscience,University of Tübingen, 3Bernstein Centre for Computational Neuroscience,University of Tübingen, 4Molecular Genetics Laboratory,University of Tübingen, 5Centre for Ophthalmology,University of Tübingen
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We describe a protocol to monitor Ca2+ dynamics in the axon terminals of cone photoreceptors using an ex-vivo slice preparation of the mouse retina. This protocol allows comprehensive studies of cone Ca2+ signaling in an important mammalian model system, the mouse.
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Kulkarni, M., Schubert, T., Baden, T., Wissinger, B., Euler, T., Paquet-Durand, F. Imaging Ca2+ Dynamics in Cone Photoreceptor Axon Terminals of the Mouse Retina. J. Vis. Exp. (99), e52588, doi:10.3791/52588 (2015).
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