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免疫组化和多标签与同一宿主物种抗体研究成人神经发生
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JoVE Journal Nörobilim
Immunohistochemistry and Multiple Labeling with Antibodies from the Same Host Species to Study Adult Hippocampal Neurogenesis
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免疫组化和多标签与同一宿主物种抗体研究成人神经发生

DOI:
10.3791/52551-v

•

09:24 min

•

April 22, 2015

•

, , ,
1Hans Berger Department of Neurology,Jena University Hospital

Bölümler

  • 00:05Başlık
  • 02:17Injection of Thymidine Analogs and Tissue Preparation
  • 03:38Sequential Multiple-Immunofluorescence with Primary Antibodies from the Same Host Species
  • 06:27Imaging and Data Analysis
  • 07:05Newborn Hippocampal Cells in Cyclin D2 Deficient Mice
  • 08:42Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

This video article illustrates a comprehensive protocol to detect and quantify all stages of adult hippocampal neurogenesis within the same tissue section. We elaborated a method to overcome the limitations of indirect multiple immunofluorescence that arise when suitable antibodies from different host species are unavailable.

Etiketler

Adult NeurogenesisImmunohistochemistryMultiple LabelingAntibodiesHippocampusNeural Stem CellsProgenitor SubtypesProliferation MarkersImmunofluorescenceConfocal MicroscopyCell QuantificationSequential Immunofluorescence

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