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Создание человека эпителиальных Enteroids и Colonoids из цельной ткани и биопсия
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Establishment of Human Epithelial Enteroids and Colonoids from Whole Tissue and Biopsy

Создание человека эпителиальных Enteroids и Colonoids из цельной ткани и биопсия

DOI:

06:33 min

March 06, 2015

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Bölümler

  • 00:05Başlık
  • 01:14Crypt Isolation from Whole Tissue
  • 03:31Crypt Culture in Basement Membrane Matrix
  • 04:27Results: Human Epithelial and Colonoids Cultured from Whole Tissue and Biopsy
  • 05:57Conclusion

Özet

Otomatik Çeviri

We describe a method to establish human enteroids from small intestinal crypts and colonoids from colon crypts collected from both surgical tissue and biopsies. In this methodological article, we present the culture modalities that are essential for the successful growth and maintenance of human enteroids and colonoids.

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