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Istituzione di umano epiteliali Enteroids e Colonoids dal tessuto intero e biopsia
JoVE Journal
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JoVE Journal Tıp
Establishment of Human Epithelial Enteroids and Colonoids from Whole Tissue and Biopsy
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Istituzione di umano epiteliali Enteroids e Colonoids dal tessuto intero e biopsia

33,961 Views

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06:33 min

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March 06, 2015

DOI:

10.3791/52483-v

DOI:
10.3791/52483-v

•

06:33 min
March 06, 2015

•

13 Views
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1Department of Pediatric General and Thoracic Surgery,Cincinnati Children’s Hospital Medical Center, 2Department of Medicine, Section of Gastroenterology and Hepatology,Baylor Tıp Fakültesi

Özet

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We describe a method to establish human enteroids from small intestinal crypts and colonoids from colon crypts collected from both surgical tissue and biopsies. In this methodological article, we present the culture modalities that are essential for the successful growth and maintenance of human enteroids and colonoids.

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Cite this Article

Mahe, M. M., Sundaram, N., Watson, C. L., Shroyer, N. F., Helmrath, M. A. Establishment of Human Epithelial Enteroids and Colonoids from Whole Tissue and Biopsy. J. Vis. Exp. (97), e52483, doi:10.3791/52483 (2015).

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