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פרוטוקול פשוט ומהיר לללא אנזימי רקמות אנושיות טריות לנתק לניתוח להחדרה לימפוציטים
Bu içeriği görüntülemek için JoVE aboneliği gereklidir. Oturum açın veya ücretsiz deneme sürümünü başlatın.
פרוטוקול פשוט ומהיר לללא אנזימי רקמות אנושיות טריות לנתק לניתוח להחדרה לימפוציטים
Bölümler
- 00:05Başlık
- 01:47Preparation of the Tissue Homogenate
- 04:15Separation and Clarification of the Tissue Supernatant
- 05:37Results: Tumor Infiltrating Lymphocyte (TIL) Subsets in the Homogenate
- 07:00Conclusion
This protocol describes the rapid non-enzymatic dissociation of fresh human tissue fragments for qualitative and quantitative assessment of CD45+ cells (lymphocytes/leukocytes) present in various normal and malignant human tissues. Additionally, the supernatant obtained from the primary tissue homogenate can be collected and stored for further analysis or experimentation.
