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ポリソームプロファイリングによる哺乳動物細胞における選択的mRNAの翻訳の評価
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October 28, 2014
DOI:
1Apoptosis Research Centre, Children’s Hospital of Eastern Ontario Research Institute and Department of Biochemistry, Microbiology and Immunology,University of Ottawa, 2Montreal Neurological Institute, 3Apoptosis Research Centre, Children’s Hospital of Eastern Ontario Research Institute and Department of Pediatrics,University of Ottawa
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The ability of cells to adapt to stress is crucial for their survival. Regulation of mRNA translation is one such adaptation strategy, providing for rapid regulation of the proteome. Here, we provide a standardized polysome profiling protocol to identify specific mRNAs that are selectively translated under stress conditions.
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Faye, M. D., Graber, T. E., Holcik, M. Assessment of Selective mRNA Translation in Mammalian Cells by Polysome Profiling. J. Vis. Exp. (92), e52295, doi:10.3791/52295 (2014).
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