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Mess Protein Stabilität in Wohnzebrafischembryonen unter Einsatz von Fluoreszenz Decay Nach Photokonversion (FDAP)
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JoVE Journal Gelişim Biyolojisi
Measuring Protein Stability in Living Zebrafish Embryos Using Fluorescence Decay After Photoconversion (FDAP)
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Mess Protein Stabilität in Wohnzebrafischembryonen unter Einsatz von Fluoreszenz Decay Nach Photokonversion (FDAP)

11,079 Views

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09:45 min

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January 28, 2015

DOI:

10.3791/52266-v

DOI:
10.3791/52266-v

•

09:45 min
January 28, 2015

•

4 Views
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1Department of Molecular and Cellular Biology,Harvard University, 2Systems Biology of Development Group,Friedrich Miescher Laboratory of the Max Planck Society

Özet

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Protein-Spiegel in Zellen und Geweben werden oft dicht durch das Gleichgewicht der Proteinproduktion und Freigabe geregelt. Verwendung Fluoreszenzabklingzeit Nach Photokonversion (FDAP) können die Clearance-Kinetik von Proteinen experimentell in vivo gemessen werden.

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Cite this Article

Rogers, K. W., Bläßle, A., Schier, A. F., Müller, P. Measuring Protein Stability in Living Zebrafish Embryos Using Fluorescence Decay After Photoconversion (FDAP). J. Vis. Exp. (95), e52266, doi:10.3791/52266 (2015).

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